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Journal / Immunology and Infection / Generating Recombinant Avian Herpesvirus Vectors…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
 

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Article DOI: 10.3791/58193-v • 12:21 min • January 7th, 2019
January 7th, 2019 •
1,2, 1, 1, 1, 1, 1, 2, 1, 1
1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

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Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.

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Keywords: CRISPR/Cas9 Recombinant Avian Herpesvirus Viral Antigens HVT Genome Recombinant Vaccines GFP Expression Cassette Cre-LoxP System Cell Sorting Chick Embryo Fibroblasts Transfection HVT Virus Stock Plaque Forming Units Trypsin-EDTA Cell Suspension Cell Density Cell Sorter GFP-expressing Cells
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