Journal
/
免疫与感染
/
Production and Titering of Recombinant Adeno-associated Viral Vectors
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Production and Titering of Recombinant Adeno-associated Viral Vectors

Production and Titering of Recombinant Adeno-associated Viral Vectors

DOI:
10.3791/3348-v

•

08:35 min

•

November 27, 2011

•

, , , ,
1School of Medical Sciences, College of Life Sciences and Medicine,University of Aberdeen, 2Translational Neuroscience Facility and Department of Physiology,School of Medical Sciences, University of New South Wales, 3Department of Biochemistry and Molecular Biophysics,Columbia University

Chapters

  • 00:05Title
  • 01:15Transfection of Viral Plasmids into Human Embryonic Kidney 293 (Hek293) Cells
  • 02:53Harvesting of rAAVs
  • 04:19Heparin Column Purification and Concentration of rAAVs
  • 06:15Titering of Viral Stocks
  • 07:25Representative Results Using rAAVs
  • 08:18Conclusion

Summary

自动翻译

自动翻译

Recombinant adeno-associated virus (rAAVs) vectors are becoming increasingly valuable for in vivo studies in animals. We describe how rAAVs can be produced in the laboratory and how these vectors can be titered to give an accurate reading of the number of infectious particles produced.

Tags

Recombinant Adeno-associated Viral VectorsAAVIn Vivo StudiesHuman Clinical TrialsTransduction ProfileImmune ResponseTransgene ExpressionGene DeliveryRAAVsSafety ProfileChimeric RAAVsAAV1AAV2Affinity ChromatographyInfectious PropertiesHeparin Column PurificationViral Titer

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Recombinant Retroviral Production and Infection of B Cells

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay

Swab Sampling Method for the Detection of Human Norovirus on Surfaces

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Read Article