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Production and Titering of Recombinant Adeno-associated Viral Vectors
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免疫与感染
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JoVE 杂志 免疫与感染
Production and Titering of Recombinant Adeno-associated Viral Vectors

Production and Titering of Recombinant Adeno-associated Viral Vectors

60,960 Views

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08:35 min

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November 27, 2011

DOI:

10.3791/3348-v

DOI:
10.3791/3348-v

•

08:35 min
November 27, 2011

•

23 Views
, , , ,
1School of Medical Sciences, College of Life Sciences and Medicine,University of Aberdeen, 2Translational Neuroscience Facility and Department of Physiology,School of Medical Sciences, University of New South Wales, 3Department of Biochemistry and Molecular Biophysics,Columbia University

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Recombinant adeno-associated virus (rAAVs) vectors are becoming increasingly valuable for in vivo studies in animals. We describe how rAAVs can be produced in the laboratory and how these vectors can be titered to give an accurate reading of the number of infectious particles produced.

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McClure, C., Cole, K. L. H., Wulff, P., Klugmann, M., Murray, A. J. Production and Titering of Recombinant Adeno-associated Viral Vectors. J. Vis. Exp. (57), e3348, doi:10.3791/3348 (2011).

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