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用 crispr/cas9 基因编辑生成重组禽流感病毒载体
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
DOI:

12:21 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:53CRISPR/Cas9-mediated Knock-in: Transfection and Infection
  • 02:14Harvesting and Purification of the HVT Recombinant Virus
  • 05:38Excision of the Fluorescent Reporter Gene via the Cre-lox System
  • 06:23Plaque Purification
  • 07:31Verification of the Recombinant HVT
  • 09:31Stability of the Recombinant Virus
  • 10:36Results: Analysis of the Generated Recombinant Avian Herpesvirus Vectors
  • 11:38Conclusion

Summary

自动翻译

火鸡疱疹病毒 (hvt) 被广泛用作生成针对多种鸟类疾病的重组疫苗的载体平台。本文介绍了一种使用集成的 nhej-crispr/cas9 和 cre-lox 系统生成重组 hvt-vectored 疫苗的简单而快速的方法。

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