Journal
/
免疫与感染
/
CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites

CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites

9,605 Views

•

14:27 min

•

November 14, 2018

DOI:

10.3791/58572-v

DOI:
10.3791/58572-v

•

14:27 min
November 14, 2018

•

3 Views
, , ,
1Heinrich Pette Institute,Leibniz Institute for Experimental Virology, 2Department of Anesthesiology,University Medical Center Hamburg-Eppendorf, 3German Center for Infection Research (DZIF)

Summary

Automatically generated

We present a genome engineering workflow for the generation of new in vitro models for HIV-1 infection that recapitulate proviral integration at selected genomic sites. Targeting of HIV-derived reporters is facilitated by CRISPR-Cas9-mediated, site-specific genome manipulation. Detailed protocols for single-cell clone generation, screening, and correct targeting verification are provided.

Explore More Videos

CRISPR-Cas9Genome EngineeringJurkat Reporter ModelsHIV-1 InfectionProviral Integration SitesGene ExpressionIn-vitro ModelsGuide RNA DesignNCBI BLASTHomology ArmsJurkat T-cell Transfection

Read Article

Cite this Article

Bialek, J. K., Walther, T., Hauber, J., Lange, U. C. CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites. J. Vis. Exp. (141), e58572, doi:10.3791/58572 (2018).

Download .ris file

Copy

Share Video

.

Copy