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Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved

Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved

18,991 Views

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12:09 min

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August 02, 2011

DOI:

10.3791/2832-v

DOI:
10.3791/2832-v

•

12:09 min
August 02, 2011

•

1 Views
, , , , ,
1Resource Ecology Group,Wageningen University, 2Section for Zoonotic Ecology and Epidemiology, School of Natural Sciences,Linnaeus University, 3Centre for Wildlife Ecology,Simon Fraser University

Summary

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A method to preserve, detect and sequence RNA from Avian Influenza Viruses was validated and extended using natural faecal samples from birds. This technique removes the necessity of maintaining a cool chain and handling of infectious viruses and can be applied in a 96-well high-throughput setup.

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Kraus, R. H., van Hooft, P., Waldenström, J., Latorre-Margalef, N., Ydenberg, R. C., Prins, H. H. Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved. J. Vis. Exp. (54), e2832, doi:10.3791/2832 (2011).

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