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Gene Editing of Primary Rhesus Macaque B Cells
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Gene Editing of Primary Rhesus Macaque B Cells

Gene Editing of Primary Rhesus Macaque B Cells

DOI:
10.3791/64858-v

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09:53 min

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February 10, 2023

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, , , , , , , ,
1Laboratory of Molecular Immunology,The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 3Laboratory of Retrovirology,The Rockefeller University, 4Howard Hughes Medical Institute,The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine,King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center,Wistar Institute

Chapters

  • 00:04Introduction
  • 00:48Preparation and Culture of Rhesus Macaque B Cells
  • 02:39Primary Rhesus Macaque B Cell Gene Editing
  • 07:36Results: Genome Editing of Primary Rhesus Macaque B Cells
  • 09:22Conclusion

Summary

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We present a method for culturing and gene editing primary rhesus macaque B cells using CRISPR/Cas9 and recombinant adeno-associated virus serotype 6 for the study of B cell therapies.

Tags

Gene EditingPrimary Rhesus Macaque B CellsCell TherapyB Cell CultureCRISPR-Cas9RAAV6Homology-directed RepairCell ActivationCell PreparationElectroporationTransductionPreclinical Research

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