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Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
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Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

28,132 Views

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21:55 min

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April 02, 2012

DOI:

10.3791/3819-v

DOI:
10.3791/3819-v

•

21:55 min
April 02, 2012

•

24 Views
, , , , , ,
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology,Heidelberg University, 2Department of Infectious Diseases, Virology,Heidelberg University

Summary

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We demonstrate the basic technique to molecularly engineer and evolve synthetic Adeno-associated viral (AAV) gene therapy vectors via DNA family shuffling. Moreover, we provide general guidelines and representative examples for selection and analysis of individual chimeric capsids with enhanced properties on target cells in culture or in mice.

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Adeno-associated Viral VectorsAAV Gene TherapyDNA Family ShufflingSynthetic VectorsTherapeutic Human Gene TransferRecombinant VectorsAAV SerotypesNatural VirusesSynthetic AAV VectorsDNA FragmentationAAV Capsid GenesPeptide DisplayHigh-throughput Method

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Kienle, E., Senís, E., Börner, K., Niopek, D., Wiedtke, E., Grosse, S., Grimm, D. Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling. J. Vis. Exp. (62), e3819, doi:10.3791/3819 (2012).

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