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Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
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JoVE 杂志 免疫与感染
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

DOI:
10.3791/3518-v

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13:47 min

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April 23, 2012

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, , , ,
1Tumour Virology Division F010,German Cancer Research Center (DKFZ), 2Inserm Unit 701,German Cancer Research Center (DKFZ)

Chapters

  • 00:05Title
  • 03:28Production of recPVs via Transfection
  • 07:09Production of recPVs via Infection
  • 11:00Results: Improved recPV Production by Adenovirus Genomic Elements
  • 13:17Conclusion

Summary

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自动翻译

Here we describe a protocol based only on cell infection, which improves the efficiency of recombinant parvovirus production by more than 100 fold in comparison to other protocols in use. This protocol relies on the use of a novel adenovirus 5-based helper containing the parvovirus VP transcription unit (Ad-VP).

Tags

Recombinant ParvovirusAdenovirus-derived SystemsRodent ParvovirusesPV GenomePromoters P4 And P38Non-structural Proteins (NS1 And NS2)Capsid Proteins (VP1 And VP2)Anticancer AgentsOncolytic AbilitiesOncosuppressive AbilitiesNS1 CytotoxicityTherapeutic TransgeneRecPVs VectorViral DNA AmplificationPackagingProducer Cells (HEK293T)PCMV-VP VectorReplication DefectiveOncotoxic ActivitiesAnti-cancer Agents

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