Journal
/
Biology
/
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
/
Um Protocolo Simples, Rápido e Parcialmente Automatizado para o Isolamento de Núcleos Únicos de Tecidos Congelados de Mamíferos para Sequenciamento de Núcleo Único
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

Um Protocolo Simples, Rápido e Parcialmente Automatizado para o Isolamento de Núcleos Únicos de Tecidos Congelados de Mamíferos para Sequenciamento de Núcleo Único

português

Automatically Generated

2,200 Views

•

01:58 min

•

July 28, 2023

DOI:

10.3791/65611-v

DOI:
10.3791/65611-v

•

01:58 min
July 28, 2023

•

26 Views
, , , ,
1Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

Explore More Videos

Single Nuclei IsolationSingle Nucleus SequencingAutomated Nuclei ExtractionStandardized ProtocolsDrug DiscoveryTranscriptomicsGene ExpressionRobotic DissociationChemical Gradient FiltrationAutomated Cell CountingMouse BrainRat KidneyCynomolgus Liver And Spleen

Read Article

Cite this Article

Stalder, L., Koechl, F., Hahn, K., Sultan, M., Prasad, M. K. A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing. J. Vis. Exp. (197), e65611, doi:10.3791/65611 (2023).

Download .ris file

Copy

Share Video

.

Copy