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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

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Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

JoVE Journal
生物学

This content is Free Access.

JoVE Journal 生物学

A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

DOI:

10.3791/65611-v

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01:58 min

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July 28, 2023

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Lucas Stalder, Fabian Koechl, Kerstin Hahn, Marc Sultan, Megana K. Prasad

¹Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

タグ

Single Nuclei Isolation Single Nucleus Sequencing Automated Nuclei Extraction Standardized Protocols Drug Discovery Transcriptomics Gene Expression Robotic Dissociation Chemical Gradient Filtration Automated Cell Counting Mouse Brain Rat Kidney Cynomolgus Liver And Spleen

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Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

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