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生物学
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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
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Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

DOI:
10.3791/65611-v

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01:58 min

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July 28, 2023

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, , , ,
1Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

Tags

Single Nuclei IsolationSingle Nucleus SequencingAutomated Nuclei ExtractionStandardized ProtocolsDrug DiscoveryTranscriptomicsGene ExpressionRobotic DissociationChemical Gradient FiltrationAutomated Cell CountingMouse BrainRat KidneyCynomolgus Liver And Spleen

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