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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
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Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
JoVE Journal
Biology
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JoVE Journal Biology
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

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2,321 Views

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01:58 min

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July 28, 2023

DOI:

10.3791/65611-v

DOI:
10.3791/65611-v

•

01:58 min
July 28, 2023

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28 Views
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1Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

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Stalder, L., Koechl, F., Hahn, K., Sultan, M., Prasad, M. K. A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing. J. Vis. Exp. (197), e65611, doi:10.3791/65611 (2023).

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