Journal
/
생물학
/
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
/
Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

2,321 Views

•

01:58 min

•

July 28, 2023

DOI:

10.3791/65611-v

DOI:
10.3791/65611-v

•

01:58 min
July 28, 2023

•

28 Views
, , , ,
1Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

Explore More Videos

Single Nuclei IsolationSingle Nucleus SequencingAutomated Nuclei ExtractionStandardized ProtocolsDrug DiscoveryTranscriptomicsGene ExpressionRobotic DissociationChemical Gradient FiltrationAutomated Cell CountingMouse BrainRat KidneyCynomolgus Liver And Spleen

Read Article

Cite this Article

Stalder, L., Koechl, F., Hahn, K., Sultan, M., Prasad, M. K. A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing. J. Vis. Exp. (197), e65611, doi:10.3791/65611 (2023).

Download .ris file

Copy

Share Video

.

Copy