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Journal
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Biologie
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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
/
Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
DOI:
10.3791/65611-v
•
01:58 min
•
July 28, 2023
•
Lucas Stalder
,
Fabian Koechl
,
Kerstin Hahn
,
Marc Sultan
,
Megana K. Prasad
1
Roche Pharma Research and Early Development, Pharmaceutical Sciences
,
Roche Innovation Centre Basel
Tags
Single Nuclei Isolation
Single Nucleus Sequencing
Automated Nuclei Extraction
Standardized Protocols
Drug Discovery
Transcriptomics
Gene Expression
Robotic Dissociation
Chemical Gradient Filtration
Automated Cell Counting
Mouse Brain
Rat Kidney
Cynomolgus Liver And Spleen
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Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
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