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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
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Un protocole simple, rapide et partiellement automatisé pour l’isolement de noyaux uniques à partir de tissus de mammifères congelés pour le séquençage d’un seul noyau
JoVE Journal
Biology
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JoVE Journal Biology
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
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Un protocole simple, rapide et partiellement automatisé pour l’isolement de noyaux uniques à partir de tissus de mammifères congelés pour le séquençage d’un seul noyau

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2,199 Views

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01:58 min

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July 28, 2023

DOI:

10.3791/65611-v

DOI:
10.3791/65611-v

•

01:58 min
July 28, 2023

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26 Views
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1Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

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Stalder, L., Koechl, F., Hahn, K., Sultan, M., Prasad, M. K. A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing. J. Vis. Exp. (197), e65611, doi:10.3791/65611 (2023).

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