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Un método de cultivo 3D de esferoides de líneas celulares embrionarias y hepáticas de pez cebra
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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
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Un método de cultivo 3D de esferoides de líneas celulares embrionarias y hepáticas de pez cebra

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2,024 Views

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08:00 min

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January 20, 2023

DOI:

10.3791/64859-v

DOI:
10.3791/64859-v

•

08:00 min
January 20, 2023

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3 Views
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1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Cell Biology,Federal University of Paraná (UFPR), 4Department of Genetics,Federal University of Paraná (UFPR)

Summary

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Aquí, presentamos un protocolo de cultivo 3D efectivo, fácil y rápido para la formación de esferoides de dos líneas celulares de pez cebra (Danio rerio): ZEM2S (embrión) y ZFL (hepatocito normal).

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de Souza, I. R., Micali Canavez, A. D. P., Schuck, D. C., Costa Gagosian, V. S., de Souza, I. R., de Albuquerque Vita, N., da Silva Trindade, E., Cestari, M. M., Lorencini, M., Leme, D. M. A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines. J. Vis. Exp. (191), e64859, doi:10.3791/64859 (2023).

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