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Une méthode de culture 3D de sphéroïdes de lignées cellulaires de poissons-zèbres embryonnaires et hépatiques
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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
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Une méthode de culture 3D de sphéroïdes de lignées cellulaires de poissons-zèbres embryonnaires et hépatiques

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2,024 Views

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08:00 min

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January 20, 2023

DOI:

10.3791/64859-v

DOI:
10.3791/64859-v

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08:00 min
January 20, 2023

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1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Cell Biology,Federal University of Paraná (UFPR), 4Department of Genetics,Federal University of Paraná (UFPR)

Summary

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Ici, nous présentons un protocole de culture 3D efficace, facile et rapide pour la formation de sphéroïdes de deux lignées cellulaires de poisson zèbre (Danio rerio): ZEM2S (embryon) et ZFL (hépatocytes normaux).

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de Souza, I. R., Micali Canavez, A. D. P., Schuck, D. C., Costa Gagosian, V. S., de Souza, I. R., de Albuquerque Vita, N., da Silva Trindade, E., Cestari, M. M., Lorencini, M., Leme, D. M. A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines. J. Vis. Exp. (191), e64859, doi:10.3791/64859 (2023).

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