/
/
A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
2,023 Views
•
08:00 min
•
January 20, 2023
DOI:
Summary
Automatically generated
Here, we present an effective, easy, and fast 3D culture protocol for the formation of spheroids of two zebrafish (Danio rerio) cell lines: ZEM2S (embryo) and ZFL (normal hepatocyte).
Read Article
Cite this Article
de Souza, I. R., Micali Canavez, A. D. P., Schuck, D. C., Costa Gagosian, V. S., de Souza, I. R., de Albuquerque Vita, N., da Silva Trindade, E., Cestari, M. M., Lorencini, M., Leme, D. M. A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines. J. Vis. Exp. (191), e64859, doi:10.3791/64859 (2023).
Copy