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Eine 3D-Kultivierungsmethode von Sphäroiden embryonaler und Leberzebrafischzelllinien
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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
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Eine 3D-Kultivierungsmethode von Sphäroiden embryonaler und Leberzebrafischzelllinien

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2,024 Views

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08:00 min

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January 20, 2023

DOI:

10.3791/64859-v

DOI:
10.3791/64859-v

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08:00 min
January 20, 2023

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3 Views
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1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Cell Biology,Federal University of Paraná (UFPR), 4Department of Genetics,Federal University of Paraná (UFPR)

Summary

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Hier stellen wir ein effektives, einfaches und schnelles 3D-Kulturprotokoll für die Bildung von Sphäroiden von zwei Zebrafisch-Zelllinien (Danio rerio) vor: ZEM2S (Embryo) und ZFL (normaler Hepatozyten).

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de Souza, I. R., Micali Canavez, A. D. P., Schuck, D. C., Costa Gagosian, V. S., de Souza, I. R., de Albuquerque Vita, N., da Silva Trindade, E., Cestari, M. M., Lorencini, M., Leme, D. M. A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines. J. Vis. Exp. (191), e64859, doi:10.3791/64859 (2023).

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