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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

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08:00 min

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January 20, 2023

DOI:

10.3791/64859-v

DOI:
10.3791/64859-v

•

08:00 min
January 20, 2023

•

3 Views
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1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Cell Biology,Federal University of Paraná (UFPR), 4Department of Genetics,Federal University of Paraná (UFPR)

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Here, we present an effective, easy, and fast 3D culture protocol for the formation of spheroids of two zebrafish (Danio rerio) cell lines: ZEM2S (embryo) and ZFL (normal hepatocyte).

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de Souza, I. R., Micali Canavez, A. D. P., Schuck, D. C., Costa Gagosian, V. S., de Souza, I. R., de Albuquerque Vita, N., da Silva Trindade, E., Cestari, M. M., Lorencini, M., Leme, D. M. A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines. J. Vis. Exp. (191), e64859, doi:10.3791/64859 (2023).

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