Journal
/
Environment
/
Método de Cultura 3D de Esferoides de Linhagens Celulares de Zebrafish Embrionárias e Hepáticas
JoVE Journal
Environment
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

Método de Cultura 3D de Esferoides de Linhagens Celulares de Zebrafish Embrionárias e Hepáticas

português

Automatically Generated

2,024 Views

•

08:00 min

•

January 20, 2023

DOI:

10.3791/64859-v

DOI:
10.3791/64859-v

•

08:00 min
January 20, 2023

•

3 Views
, , , , , , , , ,
1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Cell Biology,Federal University of Paraná (UFPR), 4Department of Genetics,Federal University of Paraná (UFPR)

Summary

Automatically generated

Aqui, apresentamos um protocolo de cultura 3D eficaz, fácil e rápido para a formação de esferoides de duas linhagens celulares de zebrafish (Danio rerio): ZEM2S (embrião) e ZFL (hepatócito normal).

Explore More Videos

3D Cell CultureZebrafish Cell LinesSpheroidsIn Vitro ModelToxicity TestingCell ViabilityTrypan BlueCell Counting96-well ULA Plate

Read Article

Cite this Article

de Souza, I. R., Micali Canavez, A. D. P., Schuck, D. C., Costa Gagosian, V. S., de Souza, I. R., de Albuquerque Vita, N., da Silva Trindade, E., Cestari, M. M., Lorencini, M., Leme, D. M. A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines. J. Vis. Exp. (191), e64859, doi:10.3791/64859 (2023).

Download .ris file

Copy

Share Video

.

Copy