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Een 3D-kweekmethode van sferoïden van embryonale en lever zebraviscellijnen
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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
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Een 3D-kweekmethode van sferoïden van embryonale en lever zebraviscellijnen

Nederlands

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2,024 Views

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08:00 min

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January 20, 2023

DOI:

10.3791/64859-v

DOI:
10.3791/64859-v

•

08:00 min
January 20, 2023

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3 Views
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1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Cell Biology,Federal University of Paraná (UFPR), 4Department of Genetics,Federal University of Paraná (UFPR)

Summary

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Hier presenteren we een effectief, eenvoudig en snel 3D-kweekprotocol voor de vorming van sferoïden van twee zebravissen (Danio rerio) cellijnen: ZEM2S (embryo) en ZFL (normale hepatocyt).

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de Souza, I. R., Micali Canavez, A. D. P., Schuck, D. C., Costa Gagosian, V. S., de Souza, I. R., de Albuquerque Vita, N., da Silva Trindade, E., Cestari, M. M., Lorencini, M., Leme, D. M. A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines. J. Vis. Exp. (191), e64859, doi:10.3791/64859 (2023).

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