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Saccharomyces cerevisiae'de Proximity Ligation ve Kantitatif PCR ile Homolog Rekombinasyon Ara Ürünlerinin Saptanması
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Genetics
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JoVE Journal Genetics
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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Saccharomyces cerevisiae'de Proximity Ligation ve Kantitatif PCR ile Homolog Rekombinasyon Ara Ürünlerinin Saptanması

Türkçe

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1,580 Views

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07:55 min

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September 11, 2022

DOI:

10.3791/64240-v

DOI:
10.3791/64240-v

•

07:55 min
September 11, 2022

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10 Views
, , ,
1Department of Microbiology & Molecular Genetics,University of California, Davis, 2Laboratory of Biology & Modeling of the Cell,École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology,University of California, Davis

Summary

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D-döngü yakalama (DLC) ve D-döngü uzatma (DLE) testleri, Saccharomyces cerevisiae'de indüklenebilir çift sarmallı bir kırılma yerinde D-döngü oluşumunu, D-döngü uzantısını ve ürün oluşumunu ölçmek için kantitatif PCR ile birlikte yakınlık ligasyonu prensibini kullanır.

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Cite this Article

Reitz, D., Savocco, J., Piazza, A., Heyer, W. Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae. J. Vis. Exp. (187), e64240, doi:10.3791/64240 (2022).

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