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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Capítulos
- 00:05Introduction
- 00:50DLC Assay
- 01:54Cell Spheroplasting, Lysis, and Restriction Site Restoration
- 03:19Restriction Enzyme Digest and Intramolecular Ligation
- 05:15Results: DLC and DLE Assay Analysis of D-Loops After DSB Induction
- 07:02Conclusion
The D-loop capture (DLC) and D-loop extension (DLE) assays utilize the principle of proximity ligation together with quantitative PCR to quantify D-loop formation, D-loop extension, and product formation at the site of an inducible double-stranded break in Saccharomyces cerevisiae.
