Detection of Homologous Recombination Intermediates <em>via</em> Proximity Ligation and Quantitative PCR in <em>Saccharomyces cerevisiae</em>

Diedre Reitz; J&#233;r&#244;me Savocco; Aur&#232;le Piazza; Wolf-Dietrich Heyer

doi:10.3791/64240

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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

1,580 Views

•

07:55 min

•

September 11, 2022

DOI:

10.3791/64240-v

DOI:

10.3791/64240-v

•

07:55 min

September 11, 2022

•

10 Views

Diedre Reitz¹, Jérôme Savocco², Aurèle Piazza², Wolf-Dietrich Heyer^1,3

¹Department of Microbiology & Molecular Genetics,University of California, Davis, ²Laboratory of Biology & Modeling of the Cell,École Normale Supérieure de Lyon, ³Department of Molecular & Cellular Biology,University of California, Davis

概要

The D-loop capture (DLC) and D-loop extension (DLE) assays utilize the principle of proximity ligation together with quantitative PCR to quantify D-loop formation, D-loop extension, and product formation at the site of an inducible double-stranded break in Saccharomyces cerevisiae.

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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

概要

Automatically generated

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