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出芽酵母における近接ライゲーションと定量PCRによる相同組換え中間体の検出

JoVE Journal
Genetics

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JoVE Journal Genetics

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

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出芽酵母における近接ライゲーションと定量PCRによる相同組換え中間体の検出

1,580 Views

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07:55 min

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September 11, 2022

DOI:

10.3791/64240-v

DOI:

10.3791/64240-v

•

07:55 min

September 11, 2022

•

10 Views

Diedre Reitz¹, Jérôme Savocco², Aurèle Piazza², Wolf-Dietrich Heyer^1,3

¹Department of Microbiology & Molecular Genetics,University of California, Davis, ²Laboratory of Biology & Modeling of the Cell,École Normale Supérieure de Lyon, ³Department of Molecular & Cellular Biology,University of California, Davis

Summary

D-ループキャプチャー(DLC)およびD-ループ伸長(DLE)アッセイは、近接ライゲーションの原理と定量的PCRを利用して、出芽酵母の誘導性二本鎖切断部位でのDループ形成、Dループ伸長、および産物形成を定量化します。

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