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Detectie van homologe recombinatietussenproducten via nabijheidsligatie en kwantitatieve PCR in saccharomyces cerevisiae
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Genetics
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JoVE Journal Genetics
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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Detectie van homologe recombinatietussenproducten via nabijheidsligatie en kwantitatieve PCR in saccharomyces cerevisiae

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1,580 Views

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07:55 min

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September 11, 2022

DOI:

10.3791/64240-v

DOI:
10.3791/64240-v

•

07:55 min
September 11, 2022

•

10 Views
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1Department of Microbiology & Molecular Genetics,University of California, Davis, 2Laboratory of Biology & Modeling of the Cell,École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology,University of California, Davis

Summary

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De D-loop capture (DLC) en D-loop extension (DLE) assays maken gebruik van het principe van proximity ligatie samen met kwantitatieve PCR om D-loopvorming, D-loop extensie en productvorming te kwantificeren op de plaats van een induceerbare dubbelstrengs breuk in Saccharomyces cerevisiae.

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Cite this Article

Reitz, D., Savocco, J., Piazza, A., Heyer, W. Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae. J. Vis. Exp. (187), e64240, doi:10.3791/64240 (2022).

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