需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
1,580 Views
•
07:55 min
•
September 11, 2022
DOI:
Summary
Automatically generated
The D-loop capture (DLC) and D-loop extension (DLE) assays utilize the principle of proximity ligation together with quantitative PCR to quantify D-loop formation, D-loop extension, and product formation at the site of an inducible double-stranded break in Saccharomyces cerevisiae.
Read Article
Cite this Article
Reitz, D., Savocco, J., Piazza, A., Heyer, W. Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae. J. Vis. Exp. (187), e64240, doi:10.3791/64240 (2022).
Copy