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Nachweis homologer Rekombinationszwischenprodukte mittels Proximityligation und quantitativer PCR in Saccharomyces cerevisiae
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Genetics
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JoVE Journal Genetics
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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Nachweis homologer Rekombinationszwischenprodukte mittels Proximityligation und quantitativer PCR in Saccharomyces cerevisiae

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1,580 Views

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07:55 min

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September 11, 2022

DOI:

10.3791/64240-v

DOI:
10.3791/64240-v

•

07:55 min
September 11, 2022

•

10 Views
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1Department of Microbiology & Molecular Genetics,University of California, Davis, 2Laboratory of Biology & Modeling of the Cell,École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology,University of California, Davis

Summary

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Die D-Loop Capture (DLC) und D-Loop Extension (DLE) Assays nutzen das Prinzip der Proximity-Ligation zusammen mit quantitativer PCR, um D-Loop-Bildung, D-Loop-Extension und Produktbildung am Ort eines induzierbaren doppelsträngigen Bruchs in Saccharomyces cerevisiae zu quantifizieren.

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Cite this Article

Reitz, D., Savocco, J., Piazza, A., Heyer, W. Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae. J. Vis. Exp. (187), e64240, doi:10.3791/64240 (2022).

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