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Developmental Biology
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固定胚胎小鼠组织以进行细胞内膜分析
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
Please note that all translations are automatically generated.
Click here for the English version.
固定胚胎小鼠组织以进行细胞内膜分析
DOI:
10.3791/64100-v
•
08:46 min
•
June 16, 2022
•
Eric T. Hall
,
Christina A. Daly
2
,
Yan Zhang
,
Miriam E. Dillard
,
Stacey K. Ogden
1
Department of Cell and Molecular Biology
,
St. Jude Children’s Research Hospital
,
2
St. Jude Graduate School of Biomedical Sciences
Chapters
00:04
Introduction
00:47
Embryo Isolation and Whole Mount Staining
03:12
Embryo Embedding, Sectioning, and Mounting
06:30
Results: Visualization of Membrane Extensions in Tissue Sections
08:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里,为胚胎的固定,免疫染色和切片提供了优化的分步方案,以检测发育中的小鼠组织中称为细胞介素的特化信号丝足类。
Tags
Cytoneme Analysis
Fixation
Light Microscopy
Endogenous Signaling Proteins
Peritoneal Cavity
Uterus
Yolk Sac
Placenta
Paraformaldehyde
HBSS
PBS
Triton
Blocking Solution
Primary Antibody
Secondary Antibody
Low Melting Point Agarose
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