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シトネム解析のための胚性マウス組織の固定
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
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シトネム解析のための胚性マウス組織の固定

日本語

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2,116 Views

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08:46 min

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June 16, 2022

DOI:

10.3791/64100-v

DOI:
10.3791/64100-v

•

08:46 min
June 16, 2022

•

8 Views
, , , ,
1Department of Cell and Molecular Biology,St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences

Summary

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ここでは、胚の固定、免疫染色、および切片化のために最適化された段階的なプロトコルが提供され、発達中のマウス組織におけるサイトネムと呼ばれる特殊なシグナル伝達糸状体を検出します。

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Cite this Article

Hall, E. T., Daly, C. A., Zhang, Y., Dillard, M. E., Ogden, S. K. Fixation of Embryonic Mouse Tissue for Cytoneme Analysis. J. Vis. Exp. (184), e64100, doi:10.3791/64100 (2022).

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