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Dissection and Culture of Mouse Embryonic Kidney
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Developmental Biology
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JoVE Journal Developmental Biology
Dissection and Culture of Mouse Embryonic Kidney

Dissection and Culture of Mouse Embryonic Kidney

DOI:
10.3791/55715-v

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08:30 min

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May 17, 2017

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1Department of Neuroscience,Uppsala University, 2Department of Organismal Biology, Evolutionary Biology Center,Uppsala University

Chapters

  • 00:05Title
  • 00:53Metanephric Rudiment Dissection at E11.5
  • 04:04Metanephric Anlagen Fixation and Staining
  • 06:07Results: Representative Ureteric Bud Branching Analyses
  • 07:48Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method for isolating and culturing metanephric rudiments from mouse embryos.

Tags

Mouse Embryonic KidneyDissectionCultureMetanephric RudimentsUreteric Bud Branching MorphologyKidney OrganogenesisTubular Branching MorphogenesisEpithelial Mesenchyme InteractionsCongenital MalformationsPolycarbonate Membrane FilterEmbryonic Day 11 1/2ForcepsDorsal AortaVentral Body WallKidney Anlagen

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