Journal
/
Biologia do Desenvolvimento
/
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

2,116 Views

•

08:46 min

•

June 16, 2022

DOI:

10.3791/64100-v

DOI:
10.3791/64100-v

•

08:46 min
June 16, 2022

•

8 Views
, , , ,
1Department of Cell and Molecular Biology,St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences

Summary

Automatically generated

Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.

Explore More Videos

Cytoneme AnalysisFixationLight MicroscopyEndogenous Signaling ProteinsPeritoneal CavityUterusYolk SacPlacentaParaformaldehydeHBSSPBSTritonBlocking SolutionPrimary AntibodySecondary AntibodyLow Melting Point Agarose

Read Article

Cite this Article

Hall, E. T., Daly, C. A., Zhang, Y., Dillard, M. E., Ogden, S. K. Fixation of Embryonic Mouse Tissue for Cytoneme Analysis. J. Vis. Exp. (184), e64100, doi:10.3791/64100 (2022).

Download .ris file

Copy

Share Video

.

Copy