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Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

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08:46 min

June 16, 2022

DOI:

08:46 min
June 16, 2022

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Summary

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Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.

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