Journal
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Biologie du développement
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Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

DOI:
10.3791/64100-v

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08:46 min

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June 16, 2022

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, , , ,
1Department of Cell and Molecular Biology,St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences

Chapitres

  • 00:04Introduction
  • 00:47Embryo Isolation and Whole Mount Staining
  • 03:12Embryo Embedding, Sectioning, and Mounting
  • 06:30Results: Visualization of Membrane Extensions in Tissue Sections
  • 08:13Conclusion

Summary

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Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.

Tags

Cytoneme AnalysisFixationLight MicroscopyEndogenous Signaling ProteinsPeritoneal CavityUterusYolk SacPlacentaParaformaldehydeHBSSPBSTritonBlocking SolutionPrimary AntibodySecondary AntibodyLow Melting Point Agarose

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