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Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

2,116 Views

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08:46 min

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June 16, 2022

DOI:

10.3791/64100-v

DOI:
10.3791/64100-v

•

08:46 min
June 16, 2022

•

8 Views
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1Department of Cell and Molecular Biology,St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences

Summary

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Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.

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Hall, E. T., Daly, C. A., Zhang, Y., Dillard, M. E., Ogden, S. K. Fixation of Embryonic Mouse Tissue for Cytoneme Analysis. J. Vis. Exp. (184), e64100, doi:10.3791/64100 (2022).

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