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发育生物学
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Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

2,116 Views

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08:46 min

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June 16, 2022

DOI:

10.3791/64100-v

DOI:
10.3791/64100-v

•

08:46 min
June 16, 2022

•

8 Views
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1Department of Cell and Molecular Biology,St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences

Summary

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Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.

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Cite this Article

Hall, E. T., Daly, C. A., Zhang, Y., Dillard, M. E., Ogden, S. K. Fixation of Embryonic Mouse Tissue for Cytoneme Analysis. J. Vis. Exp. (184), e64100, doi:10.3791/64100 (2022).

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