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Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
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JoVE Journal İmmünoloji ve Enfeksiyon
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

7,101 Views

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04:29 min

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July 01, 2022

DOI:

10.3791/63125-v

DOI:
10.3791/63125-v

•

04:29 min
July 01, 2022

•

38 Views
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1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Patoloji Bölümü,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

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Here, we present an economical and efficient method to isolate and generate high-purity bone marrow-derived dendritic cells from mice after 7 days of culture with 10 ng/mL GM-CSF/IL-4.

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Bone Marrow-derived Dendritic CellsIsolationCultureMouseBMDCCD11cCD80MSC2GM-CSFIL-4Cost-effectiveEfficient

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Tang, H., Xie, H., Wang, Z., Peng, S., Ni, W., Guo, L. Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice. J. Vis. Exp. (185), e63125, doi:10.3791/63125 (2022).

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