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Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
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JoVE Journal 免疫学と感染
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

DOI:
10.3791/63125-v

•

04:29 min

•

July 01, 2022

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, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2病理学科,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

章

  • 00:17Introduction
  • 00:37Isolation of Bone Marrow and Preparation of BM Cells
  • 02:05Induction Culture of BMDC
  • 03:11結果
  • 03:59Conclusion

概要

自動翻訳

自動翻訳

Here, we present an economical and efficient method to isolate and generate high-purity bone marrow-derived dendritic cells from mice after 7 days of culture with 10 ng/mL GM-CSF/IL-4.

タグ

Bone Marrow-derived Dendritic CellsIsolationCultureMouseBMDCCD11cCD80MSC2GM-CSFIL-4Cost-effectiveEfficient

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