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Biochemistry
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使用脂质单层方法进行电子晶体学研究的样品制备
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
Please note that all translations are automatically generated.
Click here for the English version.
使用脂质单层方法进行电子晶体学研究的样品制备
DOI:
10.3791/63015-v
•
04:22 min
•
November 20, 2021
•
Chloe D. Truong
2
,
Dewight R. Williams
,
Mary Zhu
,
Joseph Che-Yen Wang
,
Po-Lin Chiu
2
1
School of Molecular Sciences
,
Arizona State University
,
2
Center for Applied Structural Discovery, Biodesign Institute
,
Arizona State University
,
3
Eyring Materials Center
,
Arizona State University
,
4
Department of Microbiology and Immunology
,
The Pennsylvania State University
Summary
Automatic Translation
English (Original)
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Automatic Translation
几十年来，脂质单层一直被用作形成二维（2D）蛋白质晶体的基础，用于结构研究。它们在气水界面上稳定，可作为电子成像的薄支撑材料。在这里，我们介绍了为生物研究准备脂质单层的行之有效的步骤。
Tags
Lipid Monolayer
Electron Crystallography
2D Crystallization
Membrane Proteins
Protein Binding
Sample Preparation
Teflon Plate
Buffer Reservoir
Humidity Control
Carbon-coated EM Grid
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