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Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
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Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

3,543 Views

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04:22 min

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November 20, 2021

DOI:

10.3791/63015-v

DOI:
10.3791/63015-v

•

04:22 min
November 20, 2021

•

14 Views
, , , ,
1School of Molecular Sciences,Arizona State University, 2Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, 3Eyring Materials Center,Arizona State University, 4Department of Microbiology and Immunology,The Pennsylvania State University

Summary

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Lipid monolayers have been used as a foundation for forming two-dimensional (2D) protein crystals for structural studies for decades. They are stable at the air-water interface and can serve as a thin supporting material for electron imaging. Here we present the proven steps on preparing lipid monolayers for biological studies.

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Cite this Article

Truong, C. D., Williams, D. R., Zhu, M., Wang, J. C., Chiu, P. Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies. J. Vis. Exp. (177), e63015, doi:10.3791/63015 (2021).

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