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Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

English

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4,020 Views

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06:10 min

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April 08, 2021

DOI:

10.3791/62321-v

DOI:
10.3791/62321-v

•

06:10 min
April 08, 2021

•

5 Views
, ,
1Section of Structural & Synthetic Biology, Department of Infectious Diseases, Faculty of Medicine,Imperial College London, South Kensington Campus

Summary

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Sample preparation for cryo-electron microscopy (cryo-EM) is a significant bottleneck in the structure determination workflow of this method. Here, we provide detailed methods for using an easy-to-use, three-dimensionally printed block for the preparation of support films to stabilize samples for transmission EM studies.

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Cite this Article

de Martín Garrido, N., Ramlaul, K., Aylett, C. H. S. Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block. J. Vis. Exp. (170), e62321, doi:10.3791/62321 (2021).

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