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Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

JoVE 杂志
生物化学
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JoVE 杂志生物化学

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

3,497 Views

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04:22 min

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November 20, 2021

DOI:

10.3791/63015-v

DOI:

10.3791/63015-v

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04:22 min

November 20, 2021

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9 Views

Chloe D. Truong², Dewight R. Williams, Mary Zhu, Joseph Che-Yen Wang, Po-Lin Chiu²

¹School of Molecular Sciences,Arizona State University, ²Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, ³Eyring Materials Center,Arizona State University, ⁴Department of Microbiology and Immunology,The Pennsylvania State University

Tags

Lipid Monolayer Electron Crystallography 2D Crystallization Membrane Proteins Protein Binding Sample Preparation Teflon Plate Buffer Reservoir Humidity Control Carbon-coated EM Grid

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