Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Chloe D. Truong; Dewight R. Williams; Mary Zhu; Joseph Che-Yen Wang; Po-Lin Chiu

doi:10.3791/63015

Journal

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Biochemistry

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Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

JoVE Journal
Biochemistry
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JoVE Journal Biochemistry

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

3,543 Views

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04:22 min

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November 20, 2021

DOI:

10.3791/63015-v

DOI:

10.3791/63015-v

•

04:22 min

November 20, 2021

•

14 Views

Chloe D. Truong^1,2, Dewight R. Williams³, Mary Zhu¹, Joseph Che-Yen Wang⁴, Po-Lin Chiu^1,2

¹School of Molecular Sciences,Arizona State University, ²Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, ³Eyring Materials Center,Arizona State University, ⁴Department of Microbiology and Immunology,The Pennsylvania State University

Summary

Lipid monolayers have been used as a foundation for forming two-dimensional (2D) protein crystals for structural studies for decades. They are stable at the air-water interface and can serve as a thin supporting material for electron imaging. Here we present the proven steps on preparing lipid monolayers for biological studies.

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Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

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