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Genetics
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使用
埃及伊
蚊的高分辨率熔融分析进行CRISPR/Cas9诱变后的Indel检测
JoVE Journal
Genetics
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JoVE Journal
Genetics
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito
Aedes aegypti
Please note that all translations are automatically generated.
Click here for the English version.
使用
埃及伊
蚊的高分辨率熔融分析进行CRISPR/Cas9诱变后的Indel检测
DOI:
10.3791/63008-v
•
05:30 min
•
September 10, 2021
•
Bianca B. Kojin
,
Hitoshi Tsujimoto
,
Emma Jakes
,
Sarah O’Leary
,
Zach N. Adelman
1
Department of Entomology and Agrilife Research
,
Texas A&M University
Chapters
00:05
Introduction
00:33
A Gradient PCR Using Genomic DNA (gDNA) from a Single Individual
01:09
Preparation of Genomic DNA from Mosquito Legs
02:31
Perform PCR
03:35
Results: Detection of CRISPR/Cas9-Induced Indels and the Establishment of Mutant Lines in the Mosquito
Aedes aegypti
05:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文详细介绍了快速鉴定CRISPR / Cas9诱导的内嵌的方案,以及使用高分辨率熔体分析选择蚊子
埃及伊蚊
中的突变系。
Tags
CRISPR/Cas9
Indel Detection
High-resolution Melt Analysis
Aedes Aegypti
Mosquito
Primer Design
Gradient PCR
DNA Release
Thermal Melt Profile
PCR Plate
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