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Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus
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JoVE Journal Genetics
Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

DOI:
10.3791/56035-v

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05:34 min

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February 01, 2018

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1Department of Developmental and Cell Biology,University of California, Irvine

Chapters

  • 00:04Title
  • 00:30Transplantation of PGCs
  • 03:36Post-healing Care of Embryos
  • 04:39Results: CRISPR/Cas9 Leapfrogging in Xenopus
  • 05:16Conclusion

Summary

Automatic Translation

Automatic Translation

Genes essential for survival pose technical hurdles for creating mutant lines. Leapfrogging circumvents lethality by combining genome editing with primordial germ cell transplantation to create wild-type animals carrying germline mutations. Leapfrogging also permits the efficient generation of homozygous null mutants in the F1 generation. Here, the transplantation step is demonstrated.

Tags

CRISPR/Cas9LeapfroggingPrimordial Germ Cell TransplantationXenopusDevelopmental GeneticsEssential GenesEmbryogenesisBlastula StageVegetal PoleBlastoporeGraft

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