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Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
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Genetics
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JoVE Journal Genetics
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

English

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8,887 Views

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04:51 min

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November 14, 2016

DOI:

10.3791/54810-v

DOI:
10.3791/54810-v

•

04:51 min
November 14, 2016

•

2 Views
,
1Division of Biological Sciences, Section of Neurobiology,University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine,University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Summary

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Genetically-encoded histone-miniSOG induces genome-wide heritable mutations in a blue light-dependent manner. This mutagenesis method is simple, fast, free of toxic chemicals, and well-suited for forward genetic screening and transgene integration.

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Cite this Article

Noma, K., Jin, Y. Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans. J. Vis. Exp. (117), e54810, doi:10.3791/54810 (2016).

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