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Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
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Genetics
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JoVE Journal Genetics
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

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3,081 Views

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05:30 min

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September 10, 2021

DOI:

10.3791/63008-v

DOI:
10.3791/63008-v

•

05:30 min
September 10, 2021

•

1 Views
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1Department of Entomology and Agrilife Research,Texas A&M University

Summary

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This article details a protocol for rapid identification of indels induced by CRISPR/Cas9 and selection of mutant lines in the mosquito Aedes aegypti using high-resolution melt analysis.

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Cite this Article

Kojin, B. B., Tsujimoto, H., Jakes, E., O'Leary, S., Adelman, Z. N. Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti. J. Vis. Exp. (175), e63008, doi:10.3791/63008 (2021).

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