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モスキートAedes aegyptiにおける高解像度メルト分析を用いたCRISPR/Cas9突然変異誘発に続くインデル検出
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JoVE Journal Genetics
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
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モスキートAedes aegyptiにおける高解像度メルト分析を用いたCRISPR/Cas9突然変異誘発に続くインデル検出

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3,082 Views

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05:30 min

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September 10, 2021

DOI:

10.3791/63008-v

DOI:
10.3791/63008-v

•

05:30 min
September 10, 2021

•

1 Views
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1Department of Entomology and Agrilife Research,Texas A&M University

Summary

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この記事では、CRISPR/Cas9によって誘導されるインデルの迅速な同定と、高解像度の溶融分析を用いた蚊 Aedes aegypti の突然変異線の選択のためのプロトコルについて詳しく説明します。

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Cite this Article

Kojin, B. B., Tsujimoto, H., Jakes, E., O'Leary, S., Adelman, Z. N. Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti. J. Vis. Exp. (175), e63008, doi:10.3791/63008 (2021).

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