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Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
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JoVE 杂志 遗传学
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

DOI:
10.3791/63008-v

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05:30 min

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September 10, 2021

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, , , ,
1Department of Entomology and Agrilife Research,Texas A&M University

Chapters

  • 00:05Introduction
  • 00:33A Gradient PCR Using Genomic DNA (gDNA) from a Single Individual
  • 01:09Preparation of Genomic DNA from Mosquito Legs
  • 02:31Perform PCR
  • 03:35Results: Detection of CRISPR/Cas9-Induced Indels and the Establishment of Mutant Lines in the Mosquito Aedes aegypti
  • 05:02Conclusion

Summary

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This article details a protocol for rapid identification of indels induced by CRISPR/Cas9 and selection of mutant lines in the mosquito Aedes aegypti using high-resolution melt analysis.

Tags

CRISPR/Cas9Indel DetectionHigh-resolution Melt AnalysisAedes AegyptiMosquitoPrimer DesignGradient PCRDNA ReleaseThermal Melt ProfilePCR Plate

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