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Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
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September 10, 2021
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This article details a protocol for rapid identification of indels induced by CRISPR/Cas9 and selection of mutant lines in the mosquito Aedes aegypti using high-resolution melt analysis.
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Kojin, B. B., Tsujimoto, H., Jakes, E., O'Leary, S., Adelman, Z. N. Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti. J. Vis. Exp. (175), e63008, doi:10.3791/63008 (2021).
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