Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Indel Detection following CRISPR/Cas9 Mutagenesis …
JoVE Journal
Genetics

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Genetics
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
 

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Article DOI: 10.3791/63008-v • 05:30 min • September 10th, 2021
September 10th, 2021 •
1, 1, 1, 1, 1
1Department of Entomology and Agrilife Research, Texas A&M University

Capítulos

Resumo
Automatic Translation

This article details a protocol for rapid identification of indels induced by CRISPR/Cas9 and selection of mutant lines in the mosquito Aedes aegypti using high-resolution melt analysis.

Tags

Indel Detection CRISPR/Cas9 Mutagenesis High-resolution Melt Analysis Aedes Aegypti Mutants Protocol Technique Organisms Primer Design Verification HRMA Gradient PCR Master Mix Non-template Control (NTC) Template Sample Mix Cycling Parameters Thermal Melt Profiles DNA Release Reagent Dilution Buffer Genotyping Multichannel Pipet G1 Mosquitoes Wild-type Mosquitoes Hind Legs
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter